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Prior to MS Analysis, the system was calibrated not only in mass dimension but also in ion mobility by using a spiked filter with reference calibrants (lock masses) with three Agilent ESI-L Tuning Mix ions, and the collision energy was decreased linearly from 45 eV at 1/K0 = 1.30 Vs cm −2 to 27 eV at 1/K0 = 0.85 Vs cm −2
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